primerlab probe-check

CLI reference for the primerlab probe-check command

Check TaqMan probe binding efficiency and thermodynamics (v0.6.0).

Usage

primerlab probe-check --probe <SEQUENCE> [OPTIONS]

Arguments

Argument	Required	Description
--probe, -p	Yes	Probe sequence (5’→3’)
--amplicon, -a	No	Amplicon sequence for position analysis
--min-temp	No	Minimum temperature (default: 55°C)
--max-temp	No	Maximum temperature (default: 72°C)
--output, -o	No	Output file path
--format	No	Output format: text or json

Examples

Basic probe check

primerlab probe-check --probe ATGCGATCGATCGATCGATCG

Output:

═══════════════════════════════════════════════════════
                   PROBE BINDING CHECK (v0.6.0)
═══════════════════════════════════════════════════════
Probe:    ATGCGATCGATCGATCGATCG
Length:   21 bp

Results:
  Binding Tm:     65.2°C
  Grade:          A
  Quality Score:  92/100

With amplicon context

primerlab probe-check \
  --probe ATGCGATCGATCGATCGATCG \
  --amplicon ATGCGATCGATCGATCGATCGATCGATCGATCGATCGATCG

JSON output

primerlab probe-check --probe ATGCGATCGATCGATCGATCG --format json

Save to file

primerlab probe-check --probe ATGCGATCGATCGATCGATCG -o probe_result.txt

Output Fields

Field	Description
Binding Tm	Melting temperature of probe-target hybrid
Grade	Quality grade (A-F)
Quality Score	Overall score (0-100)
Position	Probe position within amplicon (if provided)

See Also

• melt-curve - SYBR melt curve prediction
• amplicon-qc - Amplicon quality check
• Probe Binding Feature