primerlab melt-curve

CLI reference for the primerlab melt-curve command

Predict SYBR Green melt curve for amplicon analysis (v0.6.0).

Usage

primerlab melt-curve --amplicon <SEQUENCE> [OPTIONS]

Arguments

Argument	Required	Description
--amplicon, -a	Yes	Amplicon sequence or FASTA file path
--output, -o	No	Output file path for plot
--format	No	Output format: text, json, svg, png
--min-temp	No	Minimum temperature (default: 65°C)
--max-temp	No	Maximum temperature (default: 95°C)

Examples

Basic melt curve prediction

primerlab melt-curve --amplicon ATGCGATCGATCGATCGATCGATCGATCGATCG

Output:

═══════════════════════════════════════════════════════
                   MELT CURVE PREDICTION (v0.6.0)
═══════════════════════════════════════════════════════
Amplicon Length:  33 bp
Predicted Tm:     72.5°C
Tm Range:         70.0 - 75.0°C
Single Peak:      ✅ Yes
Quality Score:    95/100
Grade:            A

Generate SVG plot

primerlab melt-curve \
  --amplicon ATGCGATCGATCGATCGATCGATCGATCGATCG \
  --format svg \
  --output melt_curve.svg

Generate PNG plot

primerlab melt-curve \
  --amplicon ATGCGATCGATCGATCGATCGATCGATCGATCG \
  --format png \
  --output melt_curve.png

From FASTA file

primerlab melt-curve --amplicon amplicon.fasta --format json

JSON output

primerlab melt-curve --amplicon ATGCGATCGATCGATCGATCG --format json

Output Fields

Field	Description
Predicted Tm	Primary melting temperature
Tm Range	Expected Tm variation
Single Peak	Whether curve shows single specific product
Quality Score	Overall score (0-100)
Grade	Quality grade (A-F)

Output Formats

Format	Description
text	Human-readable summary (default)
json	Full data as JSON
svg	Vector plot (no dependencies)
png	Raster plot (requires matplotlib)

See Also

• probe-check - TaqMan probe binding
• amplicon-qc - Amplicon quality check
• Melt Curve Feature