PrimerLab Documentation

Modular bioinformatics framework for automated primer and probe design

PrimerLab is a modular bioinformatics framework for automated primer and probe design.

Quick Links

• Quick Start: Design your first primers in 5 minutes.
• Guides: Step-by-step tutorials for PCR, qPCR, and more.
• Reference: CLI commands, API docs, and configuration.
• Concepts: Learn about scoring, algorithms, and quality control.

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Installation

pip install primerlab-genomic

Verify installation:

primerlab --version
primerlab health

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Key Features

Feature	Description
PCR Design	Standard primer design with quality control
qPCR Design	TaqMan and SYBR Green assay design
In-silico PCR	Virtual amplification validation
Off-target Check	BLAST-based specificity analysis
Batch Processing	Multi-sequence design
Species Specificity	Cross-reactivity testing

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Quick Example

# Create configuration
cat > my_config.yaml << 'EOF'
input:
  sequence_path: ./gene.fasta
parameters:
  tm:
    opt: 60.0
  product_size:
    min: 150
    max: 300
EOF

# Design primers
primerlab run pcr --config my_config.yaml

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Architecture

PrimerLab uses a three-tier design:

Learn more: System Architecture

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Version

Current version: v1.0.1

See Changelog for version history.

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Links

• GitHub Repository
• PyPI Package
• DeepWiki Docs